RNA-protein interaction mapping via MS2- or Cas13-based APEX targeting [Biochemistry]

RNA–protein interactions underlie a wide range of cellular processes. Improved methods are needed to systematically map RNA–protein interactions in living cells in an unbiased manner. We used two approaches to target the engineered peroxidase APEX2 to specific cellular RNAs for RNA-centered proximity biotinylation of protein interaction partners. Both an MS2-MCP...

Source: Proceedings of the National Academy of Sciences - September 7, 2020 Category: Science Authors: Shuo Han, Boxuan Simen Zhao, Samuel A. Myers, Steven A. Carr, Chuan He, Alice Y. Ting Tags: Biological Sciences Source Type: research