Diagnostic test assessment. Validation study of an alternative system to detect microsatellite instability in colorectal carcinoma.

In this study, we validated an alternative method based on capillary electrophoresis system label-free PCR (Qiaxcel system) to evaluate the MSI Bethesda Panel. We also parallel the results with a standard approach. Our data showed total concordance with the standard approach, with a highly time-efficient and easy procedure combined with high sensitivity for MSI detection. Alternative capillary electrophoresis based on label-free PCR such as the Qiaxel system is a very sensitive and specific method to detect MSI for the management of patients with colorectal cancer. This procedure is adequate and suitable in diagnostic routine for the evaluation of microsatellite repeats compared to standard procedures. PMID: 32893846 [PubMed - as supplied by publisher]
Source: Pathologica - Category: Pathology Tags: Pathologica Source Type: research