Blood-cerebrospinal fluid barrier: another site disrupted during experimental cerebral malaria caused by Plasmodium berghei ANKA.

Blood-cerebrospinal fluid barrier: another site disrupted during experimental cerebral malaria caused by Plasmodium berghei ANKA. Int J Parasitol. 2020 Aug 31;: Authors: Ngo-Thanh H, Sasaki T, Suzue K, Yokoo H, Isoda K, Kamitani W, Shimokawa C, Hisaeda H, Imai T Abstract Cerebral malaria (CM) is one of the most severe pathologies of malaria; it induces neuro-cognitive sequelae and has a high mortality rate. Although many factors involved in the development of CM have been discovered, its pathogenic mechanisms are still not completely understood. Most studies on CM have focused on the blood-brain barrier (BBB), despite the importance of the blood-cerebrospinal fluid barrier (BCSFB), which protects the brain from peripheral inflammation. Consequently, the pathological role of the BCSFB in CM is currently unknown. To examine the status of the BCSFB in CM and malaria without this pathology (non-CM), we developed a new method for evaluating the permeabilization of the BCSFB during CM in mice, using Evans blue dye and a software-assisted image analysis. Using C57BL/6J (B6) mice infected with Plasmodium berghei ANKA strain as an experimental CM model and B6 mice infected with P. berghei NK65 strain or Plasmodium yoelii as non-CM models, we revealed that the permeability of the BCSFB increased during experimental CM but not during non-CM. We observed hemorrhaging in the cerebral ventricles and hemozoin-like structures in the choroid plexus, ...
Source: International Journal for Parasitology - Category: Parasitology Authors: Tags: Int J Parasitol Source Type: research