Optimal timing for Agrobacterium-mediated DNA transformation of Trichoderma reesei conidia revealed by live cell imaging.

Optimal timing for Agrobacterium-mediated DNA transformation of Trichoderma reesei conidia revealed by live cell imaging.
Fungal Genet Biol. 2020 Aug 28;:103448
Authors: Kilaru S, Schuster M, Murray R, Steinberg G
Abstract
Trichoderma reesei is the foremost fungal producer of enzymes for industrial processes. Here, we use fluorescent live cell imaging of germinating conidia to improve Agrobacterium tumefaciens-mediated transformation (ATMT) efficiency. We define the timing of (a) morphological changes and (b) nuclear reorganisation during initial conidia germination. This reveals that conidia swell for 7h, during which nuclei undergo 2 non-synchronised mitotic divisions. Histones are recruited to the nucleus during the first 2h, suggesting that conidia enter S-phase immediately after activation. This correlates with a significantly increased ATMT efficiency at 2h after germination initiation. This finding promises to improve genetic manipulation efficiency in T. reesei.
PMID: 32866613 [PubMed - as supplied by publisher]

Source: Fungal Genetics and Biology - August 27, 2020 Category: Biology Authors: Kilaru S, Schuster M, Murray R, Steinberg G Tags: Fungal Genet Biol Source Type: research