Formulation and characterization of nanoemulsion from Alhagi maurorum essential oil and study of its antimicrobial, antibiofilm, and plasmid curing activity against antibiotic-resistant pathogenic bacteria

AbstractNanoemulsion technology is an alternative candidate to overcome antibiotic resistance in pathogenic bacteria. The aim of this research was nanoemulsion production from the essential oil ofAlhagi maurorum and the characterization of this nanostructure. Nanoemulsion of essential oil fromA. maurorum was prepared using the ionotropic gelation method and chitosan as a nano-carrier. Scanning Electron Microscopy (SEM) was used to characterize the synthesized nanoparticles. The effect of nanoemulsion on the antibacterial, antibiofilm, and plasmid curing of six antibiotic-resistant pathogenic bacteria (P. aeruginosa, E. coli, S. aureus, K. pneumonia, A. baumannii, B. cereus) was evaluated. The results of this study showed that nanoparticles had a spherical shape and smooth topology. The mean size were 172  ± 4 nm and Zeta potentials was +28.6 mv. The results of antibacterial activity confirmed that nanoemulsion of essential oil had higher inhibition against bacteria compared to free essential oil. Also, this nanoemulsion had antibiofilm activity. Minimum Inhibitory Concentration (MIC) and Minim um Bactericidal Concentration for Biofilm (MBCB) were determined for nanoemulsion against the biofilm of pathogenic bacteria. The results have shown that the MIC value forA. baumannii is 12.5  mg ml−1 and forE. coli this value is 1.75  mg ml−1. This finding means that MIC values were highest forA. baumannii and lowest forE. coli. Statistical analysis demonstrated that the ...
Source: Journal of Environmental Health Science and Engineering - Category: Environmental Health Source Type: research