CRISPR screens in the era of microbiomes.
CRISPR screens in the era of microbiomes.
Curr Opin Microbiol. 2020 Aug 25;57:70-77
Authors: Rousset F, Bikard D
Abstract
Recent advances in genomics have uncovered the tremendous diversity and richness of microbial ecosystems. New functional genomics methods are now needed to probe gene function in high-throughput and provide mechanistic insights. Here, we review how the CRISPR toolbox can be used to inactivate, repress or overexpress genes in a sequence-specific manner and how this offers diverse attractive solutions to identify gene function in high-throughput. Developed both in eukaryotes and prokaryotes, CRISPR screening technologies have already provided meaningful insights in microbiology and host-pathogen interactions. In the era of microbiomes, the versatility and the functional diversity of CRISPR-derived tools has the potential to significantly improve our understanding of microbial communities and their interaction with the host.
PMID: 32858412 [PubMed - as supplied by publisher]
Source: Current Opinion in Microbiology - Category: Microbiology Authors: Rousset F, Bikard D Tags: Curr Opin Microbiol Source Type: research
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