Enhanced KCl-mediated contractility and Ca2+ sensitization in porcine collateral-dependent coronary arteries persists after exercise training.

Enhanced KCl-mediated contractility and Ca2+ sensitization in porcine collateral-dependent coronary arteries persists after exercise training. Am J Physiol Heart Circ Physiol. 2020 Aug 28;: Authors: Heaps CL, Bray JF, Parker JL Abstract We have previously reported enhanced Ca2+ sensitivity of coronary arteries that are dependent upon collateral circulation for their blood supply. For the current study, we hypothesized that small collateral-dependent arteries would exhibit an enhanced KCl-mediated contractile response attributable to Ca2+ sensitization and increased Ca2+ channel current. Ameroid constrictors were surgically placed around the left circumflex (LCX) artery of female Yucatan miniature swine. Eight weeks post-operatively, pigs were randomized into sedentary or exercise-trained (treadmill run; 5 days/week; 14 weeks) groups. Small coronary arteries (150-300 mm luminal diameter) were isolated from myocardial regions distal to the collateral-dependent LCX and the nonoccluded left anterior descending arteries. Contractile tension and simultaneous measures of both tension and intracellular free Ca2+ levels (fura-2) were measured in response to increasing concentrations of KCl. In addition, whole-cell Ca2+ currents were also obtained. Chronic occlusion enhanced contractile responses to KCl and increased Ca2+ sensitization in collateral-dependent compared to nonoccluded arteries of both sedentary and exercise-trained pigs. In cont...
Source: American Journal of Physiology. Heart and Circulatory Physiology - Category: Physiology Authors: Tags: Am J Physiol Heart Circ Physiol Source Type: research