Isolation and genome characterization of a novel duck Tembusu virus with 74 nucleotides insertion in the 3' non-translated region.

Isolation and genome characterization of a novel duck Tembusu virus with 74 nucleotides insertion in the 3' non-translated region.
Avian Pathol. 2015 Jan 13;:1-40
Authors: Wang J, Liu W, Meng G, Zhao K, Gu J, Chen P, Cao R
Abstract
During investigations into the outbreak of duck Tembusu virus (DTMUV) infection in 2011 in China, a DTMUV strain (DTMUV-AH2011) was isolated from the affected ducks. The length of the genome of the DTMUV-AH2011 strain was found to be 11,064 nucleotides and to possess 10,278 nucleotides of one open reading frame (ORF), flanked by 94 nucleotides of the 5'non-translated region (NTR) and 692 nucleotides of the 3' non-translated region (NTR). In comparison with 5 fully sequenced TMUV genomes, the genome of DTMUV-AH2011 had 74 nucleotides insertion in the 3' NTR. Comparison of the DTMUV-AH2011 fully deduced amino acid sequences with those of other Tembusu virus strains reported recently in China showed they had a highly conserved polyprotein precursor, sharing 98.9% amino acid identities, at least. The overall divergences of amino acid substitutions were randomly distributed among viral proteins except for the protein NS4B, the protein NS4B was unchanged. Knowledge of the biological characters of DTMUV and the potential role of the insertion in the 3'NTR in RNA replication will be useful for further studies of the mechanisms of virus replication and pathogenesis.
PMID: 25582834 [PubMed - as supplied by p...

Source: Avian Pathology - January 13, 2015 Category: Pathology Authors: Wang J, Liu W, Meng G, Zhao K, Gu J, Chen P, Cao R Tags: Avian Pathol Source Type: research