Quantitative analysis of 2-hydroxyglutarate as a controversial oncometabolite in malignant gliomas.

The objective of this study was to confirm or to reject the hypothesis of the direct correlation of 2-HG concentration in tumor tissue and the results from IDH 1/IDH 2 point mutation analyses. The concentrations of 2-HG were quantified using high sensitive HPLC and Q-TOF HRMS spectrometer setup. Concurrently, the genetic mutation analyses of both IDH 1 (cytosolic) and IDH 2 (mitochondrial) were performed by the isolation of tumor tissue DNA, PCR amplification, and subsequent Sanger forward sequencing. Our results indicate that there is no definite correlation between the two as we identified cases of glioma tumors with significantly increased concentration of one or both L- and D- 2-HG but no IDH 1/2 mutations (44% 2-HG positive cases). PMID: 32853017 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/32853017?dopt=Abstract

Source: Neoplasma - August 27, 2020 Category: Cancer & Oncology Authors: Bystrický P, Kašubová I, Richterová R, Lasabová Z, Kolarovszki B Tags: Neoplasma Source Type: research