Prenatal Exposure to E-Cigarette Aerosols Leads to Sex-dependent Pulmonary Extracellular Matrix Remodeling and Myogenesis in Offspring Mice.

Prenatal Exposure to E-Cigarette Aerosols Leads to Sex-dependent Pulmonary Extracellular Matrix Remodeling and Myogenesis in Offspring Mice. Am J Respir Cell Mol Biol. 2020 Aug 27;: Authors: Wang Q, Sundar IK, Blum JL, Ratner JR, Lucas JH, Chuang TD, Wang Y, Liu J, Rehan VK, Zelikoff JT, Rahman I Abstract Electronic cigarette (e-cig) vaping is a serious concern, since many pregnant women who vape consider it safe. However, little is known about the harmful effects of prenatal e-cig exposure on the adult offspring, especially on extracellular matrix (ECM) deposition and myogenesis in the lungs of offspring. We evaluated the biochemical and molecular implications of maternal exposure during pregnancy to e-cigs on the adult offspring of both sexes with a particular focus on pulmonary ECM remodeling and myogenesis. Pregnant CD-1 mice were exposed to e-cig aerosol with or without nicotine, throughout gestation, and lungs were collected from adult male and female offspring. Compared to the air group, female mice exposed to e-cig aerosols, with or without nicotine, demonstrated increased lung protein abundance of LEF-1, fibronectin, and E-cadherin, while altered E-cadherin and PPARĪ³ levels were observed only in males exposed to e-cig aerosols with nicotine. Moreover, lipogenic/myogenic mRNAs were dysregulated in adult offspring in a sex-dependent manner. PAI-1, one of the ECM regulators, was significantly increased in females exposed prena...
Source: American Journal of Respiratory Cell and Molecular Biology - Category: Molecular Biology Authors: Tags: Am J Respir Cell Mol Biol Source Type: research