Sensitive determination of NT ‐proBNP for diagnosing abdominal aortic aneurysms incidence on interdigitated electrode sensor

Diagnosing abdominal aortic aneurysms incidence on inderdigitated electrode sensor. AbstractAbdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health ‐related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immediate treatment. Blood‐based biomarker helps to diagnose AAA and to monitor the condition after AAA surgery. N‐terminal pro‐B‐type natriuretic pepti de (NT‐proBNP) is a hormone produced in the heart in small quantities and increased when the heart needs to work harder. NT‐proBNP was proved to be strongly linked with AAA incidence. Moreover, quantifying the level of NT‐proBNP helps to determine the risk factors on cardiovascular system afte r the surgery. This work is quantifying the NT‐proBNP on interdigitated electrode sensor by using NT‐proBNP binding aptamer. The detection limit of NT‐proBNP was calculated as 1 pg/mL on a linear regression curve [y = 0.2148x + 0.8849;R² = 0.9049]. The linear range with dose‐dependent analysis was from 0.01 until 100 ng/mL. Moreover, the control experiment with complementary aptamer sequence did not show the current signal, specifying the detection of NT‐proBNP. This research benefits to identify the heart condition of pat ient after the removal of AAA.
Source: Biotechnology and Applied Biochemistry - Category: Biochemistry Authors: Tags: Original Article Source Type: research