An enzyme immunoassay for determining albumin in human urine samples using an ultra-microanalytical system.

An enzyme immunoassay for determining albumin in human urine samples using an ultra-microanalytical system. J Immunoassay Immunochem. 2020 Aug 16;:1-17 Authors: Del Valle R, Cazanave Mora JM, Carrazana San Martín NL, Hernández Pérez L, Legrá Torres ME, López Cisneros R, Ordaz Ramos RP, Rodríguez AM, Valdivia Álvarez I Abstract Microalbuminuria is a term to describe a moderate increase in the level of albumin in urine. It is an important prognostic marker for kidney damage in diseases such as diabetes mellitus and hypertension. A simple sandwich-type ultramicroELISA assay (UMELISA) has been developed for the measurement of albumin in human urine samples. Strips coated with a high affinity monoclonal antibody directed against albumin are used as solid phase, to ensure the specificity of the assay. The albumin assay was completed in 1 hr and 30 min, with a measuring range of 1.44-200 ng/mL. The intra- and inter-assay coefficients of variation were 3.98-4.35% and 7.59-8.92%, respectively, depending on the albumin concentrations evaluated. Percentage recovery ranged from 94.26 to 98.50%. Regression analysis showed a good correlation with the commercial quantitative turbidimetric test Microalbumin-turbilatex (n = 240, r = 0.994, p < .01). The analytical performance characteristics of our UMELISA MICROALBUMINA endorse its use for the quantification of albumin in human urine samples. This test will make a cost-effective diag...
Source: Journal of Immunoassay and Immunochemistry - Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research