The type II phosphoinositide 4-kinase FgLsb6 is important for the development and virulence of Fusarium graminearum.

In this study, we characterized the type II PI4K protein FgLsb6 in F. graminearum, a homolog of Lsb6 in Saccharomyces cerevisiae. Unlike Lsb6, FgLsb6 localizes to the vacuoles and endosomes. The ΔFglsb6 mutant displayed defects in vegetative growth, deoxynivalenol (DON) production and pathogenicity. Furthermore, the ΔFglsb6 deletion mutant also exhibited increased resistance to osmotic, oxidative and cell wall stresses. Further analyses of the ΔFglsb6 mutant showed that it was defective in the generation of PI4P on endosomes and endocytosis. Collectively, our data suggest that the decreased vegetative growth and pathogenicity of ΔFglsb6 was due to the conservative roles of FgLsb6 in the generation of PI4P on endosomes and endocytosis.
PMID: 32800918 [PubMed - as supplied by publisher]

Source: Fungal Genetics and Biology - August 11, 2020 Category: Biology Authors: Chen L, Zhang L, Mei X, Wang C, Guo Z, Li L, Li B, Liang Y, Zou S, Dong H Tags: Fungal Genet Biol Source Type: research