An Isothermal Method for Sensitive Detection of Mycobacterium tuberculosis Complex Using Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a Cis and Trans Cleavage

Tuberculosis is one of the most serious infectious diseases, resulting in death worldwide. Traditional detection methods are not enough to meet the clinical requirements of rapid diagnosis, high specificity, and high sensitivity. Fast, sensitive, and accurate detection of Mycobacterium tuberculosis (MTB) is urgently needed to treat and control tuberculosis disease. Clustered regularly interspaced short palindromic repeats (CRISPR) –associated proteins (Cas12a) exhibit strong nonspecific degradation ability of exogenous single-strand nucleic acids (trans cleavage) after specific recognition of target sequence.

Source: Journal of Molecular Diagnostics - May 25, 2020 Category: Pathology Authors: Haipo Xu, Xiaolong Zhang, Zhixiong Cai, Xiuqing Dong, Geng Chen, Zhenli Li, Liman Qiu, Lei He, Bin Liang, Xiaolong Liu, Jingfeng Liu Tags: Regular article Source Type: research