Amplicon-Based Next-Generation Sequencing for Detection of Fungi in Formalin-Fixed, Paraffin-Embedded Tissues: Correlation with Histopathology and Clinical Applications

Invasive fungal infections are increasing in prevalence due to an expanding population of immunocompromised individuals. To reduce morbidity and mortality, it is critical to accurately identify fungal pathogens to guide treatment. Current methodologies rely on histopathology, fungal culture, and serology, which are often insufficient for diagnosis. Here we describe the use of a lab-developed internal transcribed spacer (ITS) targeted amplicon-based Next Generation Sequencing (NGS) assay for the identification of fungal etiology in fungal stain positive FFPE tissues by using Illumina MiSeq.

Source: Journal of Molecular Diagnostics - July 28, 2020 Category: Pathology Authors: Paige M.K. Larkin, Katy L. Lawson, Deisy A. Contreras, Catherine Q. Le, Marisol Trejo, Susan Realegeno, Evann E. Hilt, Sukantha Chandrasekaran, Omai B. Garner, Gregory A. Fishbein, Shangxin Yang Source Type: research