Arachis hypogaea PGPR isolated from Argentine soil modifies its lipids components in response to temperature and salinity

Publication date: Available online 3 January 2015 Source:Microbiological Research Author(s): Natalia S. Paulucci , Lucas A. Gallarato , Yanina B. Reguera , Julio C. Vicario , Adriana B. Cesari , Mirta B. García de Lema , Marta S. Dardanelli The aim of this work was to clarify the mechanism related to plant growth promoting of a bacterial strain (L115) isolated from Arachis hypogaea rhizospheres and the effects of high growth temperature and salinity on phospholipids and fatty acids composition. L115 was isolated from peanut rhizospheres and identified according to the sequence analysis of the 16S rRNA gene. Phenotypic, metabolic and plant growth promoting rhizobacteria (PGPR) characteristics of L115 were tested. Inoculation test in plant growth chamber was performed. In addition, L115 was exposed to a 37°C and 300mM NaCl and phospholipids and fatty acid composition were evaluated. L115 strain was identified as Ochrobactrum intermedium and was able to increase the peanut shoot and root length as well as dry weight, indicating a PGPR role by being able to produce indole acetic acid and siderophores and present ACC deaminase activity. In addition, L115 showed tolerance to both high growth temperature and 300mM NaCl. The most striking change was a decreased percentage of 18:1 fatty acid and an increase in 16:0 and 18:0 fatty acids, under high growth temperature or a combination of increased temperature and salinity. The most important change in phospholipid levels wa...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research