An indisputable role of NHE8 in mucosal protection.

An indisputable role of NHE8 in mucosal protection. Am J Physiol Gastrointest Liver Physiol. 2020 Aug 05;: Authors: Bernardazzi C, Xu H, Tong H, Laubitz D, Figliuolo da Paz V, Curiel L, Ghishan FK Abstract The loss of the intestinal Na+/H+ exchanger isoform 8 (NHE8) results in an ulcerative colitis-like condition with reduction of mucin production and dysbiosis, indicating that NHE8 plays an important role in intestinal mucosal protection. The aim of this study was to investigate the potential rebalance of the altered microbiota community of NHE8-deficient mice via fecal microbiota transplantation (FMT) and feeding probiotic VSL#3. We also aimed to stimulate mucin production by sodium butyrate administration via enema. Data from 16S rRNA sequencing showed that loss of NHE8 contributes to colonic microbial dysbiosis with reduction of butyrate-producing bacteria. FMT increased bacterial adhesion in the colon in NHE8KO mice. PAS stain and qPCR showed no changes in mucin production during FMT. In mice treated with probiotic VSL#3, a reduction of Lactobacillus and SFB in NHE8KO mouse colon was detected, and an increase in goblet cell theca were observed. In NHE8KO mice receiving sodium butyrate (NaB), 1mM NaB stimulated Muc2 expression without changing goblet cell theca, but 10mM NaB induced a significant reduction of goblet cell theca without altering Muc2 expression. Furthermore, 5mM and 10mM NaB treated HT29-MTX cells displayed increas...
Source: American Journal of Physiology. Gastrointestinal and Liver Physiology - Category: Physiology Authors: Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research