Detection of beta-lactamase production in clinical Prevotella species by MALDI-TOF MS method.

Detection of beta-lactamase production in clinical Prevotella species by MALDI-TOF MS method. Anaerobe. 2020 Aug 05;:102240 Authors: Toprak NU, Akgul O, Sóki J, Soyletir G, Nagy E, ESCMID Study Group for Anaerobic Infections (ESGAI) Abstract Penicillins, can be used in treatment of infections due to Prevotella species if they are susceptible to penicillin. Early and accurate preliminary detection of β-lactamase-producing isolates is crucial for treatment of infection. The aim of this study was to determine β-lactamase-producing Prevotella species by MALDI-TOF MS and screen them for the presence of cfxA gene, responsible for β-lactamase production. A total of 500 clinically relevant Prevotella isolates, collected from 13 countries for the previous European antibiotic resistance surveillance study, were tested. Susceptibility testing was performed against ampicillin and ampicillin/sulbactam by Etest methodology. EUCAST guidelines were used for susceptibility interpretations; the isolates with MIC value ≤ 0.5 for ampicillin were considered susceptible and >2 resistant. All Prevotella isolates, were tested for detection of β-lactamase activity by MALDI-TOF MS (Vitek® MS Research Use Only) system and the presence of the cfxA gene by PCR method. The susceptibility levels of the isolates to ampicillin/sulbactam and ampicillin were 99.6% and 43.4%, respectively. A total 59% of isolates presented β-lactamase activity and 60.8%...
Source: Anaerobe - Category: Microbiology Authors: Tags: Anaerobe Source Type: research