FnCas12a/crRNA assisted dumbbell-PCR detection of IsomiRs with terminal and inner sequence variants.

FnCas12a/crRNA assisted dumbbell-PCR detection of IsomiRs with terminal and inner sequence variants.
Chem Commun (Camb). 2020 Jul 30;:
Authors: Wang J, Bing T, Zhang N, Liu X, Shangguan D
Abstract
Herein, we report a FnCas12a/crRNA assisted Dumbbell-PCR method for the detection of isomiRs with double specificity and magnification. The single nucleotide variant of isomiRs in terminals and/or inner sequence could be discriminated by this strategy. Using this method, let-7a isomiRs in MCF-7 and MCF-7R cell lines were analyzed.
PMID: 32729588 [PubMed - as supplied by publisher]

Source: Chemical Communications - July 29, 2020 Category: Chemistry Authors: Wang J, Bing T, Zhang N, Liu X, Shangguan D Tags: Chem Commun (Camb) Source Type: research