Analysis of the proteins secreted by Trichoderma harzianum P49P11 under carbon-limited conditions.

Analysis of the proteins secreted by Trichoderma harzianum P49P11 under carbon-limited conditions. J Proteomics. 2020 Jul 28;:103922 Authors: Gelain L, da Cruz Pradella JG, da Costa AC, van der Wielen L, van Gulik WM Abstract The wild type strain Trichoderma harzianum was able to synthesize enzymes that can catalyse the hydrolysis of p-nitrophenyl-β-D-glucopyranoside (PNPGase) in glucose-limited chemostat cultures. Fructose/glucose and sucrose conditions provided low levels of PNPGase activity. To investigate whether under these conditions other enzymes were produced, a shotgun proteomics analysis of their supernatants was performed. The analysis has indicated that the different carbon sources used influenced the amounts of proteins secreted including 1,3-beta-glucanosyltransferase, alpha-1,2-mannosidase, alpha-galactosidase and glucan 1,3-beta-glucosidase. The analysis has also suggested the presence of beta-glucosidase, which could also be represented by PNPGase activity. Intracellular metabolites were quantified during PNPGase production for the condition using 20 g/L of glucose in the feed and differences were observed, indicating that intracellular glucose could be inhibiting PNPGase production. SIGNIFICANCE: This work shows that sugars such as glucose, fructose/glucose and sucrose can be used as substrates for the continuous synthesis of different enzymes under carbon-limited conditions by Trichoderma harzianum. As far as we...
Source: Journal of Proteomics - Category: Biochemistry Authors: Tags: J Proteomics Source Type: research