Characterization of differentially expressed plasma proteins in patients with acute myocardial infarction.
Characterization of differentially expressed plasma proteins in patients with acute myocardial infarction.
J Proteomics. 2020 Jul 28;:103923
Authors: Pan Y, Wang L, Xie Y, Tan Y, Chang C, Qiu X, Li X
Abstract
Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide. Novel biomarkers are needed to identify NSTEMI in AMI patients. The study objective was to use proteomics to identify novel plasma biomarkers for STEMI and NSTEMI patients. iTRAQ analysis was performed on pooled samples from 8 healthy controls and 12 STEMI and 12 NSTEMI patients. Bioinformatics analysis identified 95 differentially expressed proteins that were differentially expressed in the plasma of AMI patients and healthy controls; 28 of these proteins were found in STEMI/Con (22 upregulated and 6 downregulated), 48 in NSTEMI/Con (12 upregulated and 36 downregulated), and 44 in NSTEMI/STEMI (11 upregulated and 33 downregulated). Protein network analysis was then performed using STRING software. Functional analysis revealed that the identified plasma proteins were mainly involved with carbon metabolism, toll-like receptor signaling pathway, and hypertrophic cardiomyopathy. Nine of the proteins (SSA1, MDH1, FCN2, GPI, S100A8, LBP, vinculin, VDBP, and RBP4) that changed levels during AMI progression were further validated by ELISA. The constructed plasma proteome could reflect the AMI pathogenesis molecular mechanisms and provide a m...
Source: Journal of Proteomics - Category: Biochemistry Authors: Pan Y, Wang L, Xie Y, Tan Y, Chang C, Qiu X, Li X Tags: J Proteomics Source Type: research
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