Development of an SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus.

In this study, an SYBR Green I-based quantitative real-time PCR assay was established for CaKoV detection. Specific primers targeting a highly conserved region of the CaKoV 3D gene were developed. After optimization, the method detected a minimum of 1 × 101 copies/µL with high specificity, stability, and repeatability. Moreover, the entire process only required approximately 1.5 h for completion. Our results were supported by those obtained for clinical samples, in which our developed method was successfully applied. The newly established real-time PCR is a rapid, sensitive, speciﬁc, and repeatable method for the quantitative detection of CaKoV and can, therefore, be used in epidemiological studies. PMID: 32730841 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/32730841?dopt=Abstract

Source: Journal of Virological Methods - July 26, 2020 Category: Virology Authors: Wang Y, Cui Y, Li Y, Zhang D, Sun J, Guo X, Liu G, Jiang S, Li Y Tags: J Virol Methods Source Type: research