Maternal-fetal Blood Major Crossmatching in Merino Sheep.

Maternal-fetal Blood Major Crossmatching in Merino Sheep.
Comp Med. 2020 Jul 29;:
Authors: Musk GC, Usuda H, Kershaw H, Kemp MW, Sharp CR
Abstract
To determine the incidence of ex vivo incompatibility between ovine maternal RBCs and fetal plasma, we performed cross-matching of blood samples from ewes and from lambs delivered by cesarean section. Twenty-one date-mated singleton pregnant Merino ewes were anesthetized for cesarean delivery of the fetus. At the time of delivery, paired maternal and fetal blood samples were collected and subsequently separated for storage as packed red blood cells and fresh frozen plasma. Gel column major cross matching was performed within 2 wk. All fetus-dam crossmatches were major crossmatches, combining fetal (recipient) plasma with dam (donor) RBCs. 172 individual dam-dam cross matches were performed. Two of these testswere incompatible (1.2%). In addition, 19 fetal blood samples collected immediately after cesarean delivery were crossmatchedwith 21 maternal samples to generate 174 maternal-fetal individual cross matches. No maternal-fetal incompatibility reactionswere observed. The results of this study demonstrate that all maternal donors and fetal recipients were compatible. Inaddition, the incidence of an incompatible crossmatch between adult ewes was 1.2%. These data suggest that lambs may notbe born with antibodies against other blood types, but rather may acquire such antibodies at some time d...

Source: Comparative Medicine - July 28, 2020 Category: Zoology Authors: Musk GC, Usuda H, Kershaw H, Kemp MW, Sharp CR Tags: Comp Med Source Type: research