Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery.
Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery.
Chem Commun (Camb). 2020 Jul 28;:
Authors: Lu X, Wu X, Wu T, Han L, Liu J, Ding B
Abstract
A terminal-closed linear gene with strong exonuclease resistance and serum stability was successfully constructed by polymerase chain reaction (PCR) with an α-l-threose nucleic acid (TNA) loop modified primer pair, which can be used as an efficient gene expression system in eukaryotic cells for gene delivery.
PMID: 32720666 [PubMed - as supplied by publisher]
Source: Chemical Communications - Category: Chemistry Authors: Lu X, Wu X, Wu T, Han L, Liu J, Ding B Tags: Chem Commun (Camb) Source Type: research