PGF2 α stimulates 10-pS Cl channel and thiazide-sensitive Na-Cl cotransporter (NCC) in distal convoluted tubule.

PGF2α stimulates 10-pS Cl channel and thiazide-sensitive Na-Cl cotransporter (NCC) in distal convoluted tubule. Am J Physiol Renal Physiol. 2020 Jul 27;: Authors: Wang LJ, Xiao Y, Fang J, Wang JL, Zhang H, Meng XX, Gong RL, Gu R Abstract We used patch-clamp and western blot to test whether PGF2α stimulates the basolateral 10-pS Cl channel and thiazide-sensitive Na-Cl cotransporter (NCC) in distal convoluted tubule (DCT) via FP receptor (FP-R). The single-channel and whole cell recordings demonstrated that PGF2α stimulated the 10-pS Cl channel in DCT. The stimulatory effect of PGF2α on the Cl channel was mimicked by FP-R agonist, Latanoprost, but was abrogated by blocking FP-R with AL8810. Also, the effect of PGF2α on the Cl channel in the DCT was recapitulated by stimulating protein kinaseC (PKC) but was blocked by inhibiting PKC. Furthermore, the inhibition of p38-mitogen-activated protein kinase (MAPK) but not ERK blocked the effect of PGF2α on 10-pS Cl. Inhibition of NADPH oxidase (NOX) also abrogated the stimulatory effect of PGF2α on the 10-pS Cl channel while adding 10μM H2O2 mimicked the stimulatory effect of PGF2α on the 10-pS Cl channel. Moreover, superoxide-related-species may mediate the stimulatory effect of PGF2α on the 10-pS Cl channel because the stimulatory effect of PGF2α and H2O2 was not additive. Western blot showed that infusion of PGF2α in vivo not only increased the expression of FP-R, but also incre...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research