Virtual Measurements of Paracellular Permeability and Chronic Inflammation Via Color Coded Pixel-wise T1 Mapping.

Virtual Measurements of Paracellular Permeability and Chronic Inflammation Via Color Coded Pixel-wise T1 Mapping. Am J Physiol Renal Physiol. 2020 Jul 27;: Authors: Singh N, Zabbarova I, Ikeda Y, Maranchie J, Chermansky C, Foley LM, Hitchens TK, Yoshimura N, Kanai AJ, Kaufman J, Tyagi P Abstract To assess whether quantitative T1 relaxometry can measure permeability, assess chronic inflammation and mural thickening of mouse bladder wall. Adult female C57BL6 mice unexposed to radiation (controls) or 40 weeks post-irradiation10Gy were scanned at 9.4T before and after instillation (0.1mL) of aqueous, novel contrast mixture containing 4mM Gadobutrol and 5mM Ferumoxytol. Rapid Acquisition with Refocused Echo (RARE) sequence with variable Repetition Times (TR) generated pixel wise map of T1 relaxation times for segmented bladder wall layers from voxel-wise, nonlinear least square data fitting of TR dependent signal intensity acquired with TR array of 0.4-10s followed by the histology of harvested bladder. Significant differences between pre-contrast and post contrast T1 (DT1) noted in urothelium and lamina propria of both groups but only in detrusor of irradiated group (p<0.001; Two-way ANOVA). Nearly two-fold higher Gadobutrol permeability (550 ± 73μM vs 294 ± 160μM; p<0.01) derived as per 1/DT1 = r1. [C] in urothelium of irradiated group (0.75± 0.04mm vs 0.44± 0.08mm; p<0.001). Inflammation and bladder wall thickening predi...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research