Proactive preparedness for Cat Que virus: An Orthobunyavirus existing in India.

Proactive preparedness for Cat Que virus: An Orthobunyavirus existing in India.
Indian J Med Res. 2020 Jun;151(6):571-577
Authors: Shete A, Yadav PD, Gokhale M, Jain R, Pardeshi P, Majumdar T, Mourya DT
Abstract
Background & objectives: The presence of Cat Que virus (CQV) in Culex mosquitoes and pigs has been reported in China and Vietnam. Due to the spread of similar species of the Culex mosquitoes in India, there is a need to understand the replication kinetics of this virus in mosquito models. As a part of preparedness and to identify the presence of this CQV in humans and swine, this study was carried out to develop diagnostic tests.
Methods: Serological and molecular diagnostic assays were developed for testing the mosquito population, human and swine serum samples. In this line, RNA-dependent RNA polymerase (L), glycoprotein (M) and nucleocapsid (S) genes-based reverse transcription-polymerase chain reaction (RT-PCR) assays were developed for CQV. Real-time RT-PCR was used for screening of retrospectively collected human serum samples (n=1020) with acute febrile illness during 2014-2017. Simultaneously, an in-house anti-CQV swine and human IgG ELISAs were also developed to detect anti-CQV IgG antibody. Human serum samples (n=883) with post-onset of disease (POD) >4 days and swine serum samples (n=459) were tested for the presence of anti-CQV IgG antibodies. CQV NIV 612,045 isolate was used for susceptibility and ...

Source: Indian J Med Res - May 31, 2020 Category: Research Authors: Shete A, Yadav PD, Gokhale M, Jain R, Pardeshi P, Majumdar T, Mourya DT Tags: Indian J Med Res Source Type: research