An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs.

An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs.
Neural Plast. 2020;2020:3570732
Authors: Wang Z, Qian M, Wang Q, Liu H, Wu H, Huang Z
Abstract
The prestin-based active process in the mammalian outer hair cells (OHCs) is believed to play a crucial role in auditory signal amplification in the cochlea. Prestin belongs to an anion transporter family (SLC26A). It is densely expressed in the OHC lateral plasma membrane and functions as a voltage-dependent motor protein. Analog genes can be found in the genome of nonmammalian species, but their functions in hearing are poorly understood. In the present study, we used the gerbil prestin sequence as a template and identified an analog gene in the bullfrog genome. We expressed the gene in a stable cell line (HEK293T) and performed patch-clamp recording. We found that these cells exhibited prominent nonlinear capacitance (NLC), a widely accepted assay for prestin functioning as a motor protein. Upon close examination, the key parameters of this NLC are comparable to that conferred by the gerbil prestin, and nontransfected cells failed to display NLC. Lastly, we performed patch-clamp recording in HCs of all three hearing organs in bullfrog. HCs in both the sacculus and the amphibian papilla exhibited a capacitance profile that is similar to NLC while HCs in the basilar papilla showed no sign of NLC. Whether or not this NLC-like capacitance change is involved in auditory ...

Source: Neural Plasticity - July 29, 2020 Category: Neurology Authors: Wang Z, Qian M, Wang Q, Liu H, Wu H, Huang Z Tags: Neural Plast Source Type: research