Using RNA Tags for Multicolor Live Imaging of Chromatin Loci and Transcription in Drosophila Embryos.

Using RNA Tags for Multicolor Live Imaging of Chromatin Loci and Transcription in Drosophila Embryos.
Methods Mol Biol. 2020;2166:373-384
Authors: Chen H, Gregor T
Abstract
Elucidating the biological implications of higher order chromatin architectures in animal development requires simultaneous, quantitative measurements of chromatin dynamics and transcriptional activity in living specimen. Here we describe a multicolor labeling and live imaging approach in embryos of the fruit fly Drosophila melanogaster. The method allows simultaneous measurement of movements of specific loci and their transcriptional activity for developmental genes, enabling new approaches to probe the interaction between 3D chromatin architecture and regulation of gene expression in development.
PMID: 32710421 [PubMed - in process]

Source: Mol Biol Cell - July 28, 2020 Category: Molecular Biology Authors: Chen H, Gregor T Tags: Methods Mol Biol Source Type: research