Nisin influence on the expression of Listeria monocytogenes surface proteins.

Nisin influence on the expression of Listeria monocytogenes surface proteins. J Proteomics. 2020 Jul 21;:103906 Authors: Stincone P, Miyamoto KN, Timbe PPR, Lieske I, Brandelli A Abstract In this work, a comparative analysis of the peripheral cell component (PCC) proteins of Listeria monocytogenes was carried out. The study was conducted on two set of samples consisting of bacteria treated with sub-lethal concentration of nisin and untreated bacteria as control. PCC proteins were extracted by Tris-Urea-EDTA treatment and then subjected to trypsin digestion and mass spectrometry analysis. The whole cell proteome was analyzed through label-free quantitative proteomics approach. Proteomic analysis was carried out using OrbiTrap Mass Spectrometer coupled to nanoflow liquid chromatography. The treatment with sub-lethal nisin concentration resulted in 62 up regulated and 97 down regulated proteins compared to untreated samples. Using PSORTb 3.0, 19 and 18 surface proteins were detected among the up regulated and down regulated proteins, respectively. Proteins related with increased biofilm formation by L. monocytogenes, such as moonlight proteins of the pyruvate dehydrogenase complex and flagellin-related proteins, were identified as up regulated surface proteins. Proteins associated with virulence of L. monocytogenes, including listeriolysin O, internalin B and actin assembly-inducing protein, were detected among the down regulated protei...
Source: Journal of Proteomics - Category: Biochemistry Authors: Tags: J Proteomics Source Type: research