Development of Spectrophotometric and Validated Stability-Indicating RP-HPLC Methods for Simultaneous Determination Of Ephedrine HCL, Naphazoline HCL, Antazoline HCL, and Chlorobutanol In Pharmaceutical Pomade Form.

CONCLUSION: The LOD values were found to be 3.95, 0.19, 0.92 and 0.96 µg mL-1 for EPD HCl, NPZ HCl, ANT HCl, and CLO in spectrophotometric method, respectively. The linear ranges in the RP-HPLC method were 8.2-24.36 µg mL-1, 0.083 - 0.75 µg/mL, 2.01-7.5 µg mL-1 and 2.89 - 24.4 µg mL-1 for EPD HCl, NPZ HCl, ANT HCl, and CLO, respectively. The LOD values in the validation studies were 2.7, 0.025, 0.66 and 0.86 µg mL-1 for EPD HCl, NPZ HCl, ANT HCl, and CLO in RP-HPLC method respectively. The results of the spectrophotometric and chromatographic methods were compared and no differences were found between the two methods.
PMID: 32691707 [PubMed - as supplied by publisher]

Source: Combinatorial Chemistry and High Throughput Screening - July 18, 2020 Category: Chemistry Authors: Dindar ÇK, Erkmen C, Uslu B, Göğer NG Tags: Comb Chem High Throughput Screen Source Type: research