Apolipoprotein L1 is transcriptionally regulated by SP1, IRF1 and IRF2 in hepatoma cells

AbstractApolipoprotein L1 (APOL1) participates in lipid metabolism. Here, we investigate the mechanisms regulatingAPOL1 gene expression in hepatoma cells. We demonstrate that the −80 nt to +31 nt region of theAPOL1 promoter, which contains one SP transcription factor ‐binding GT‐Box and an interferon regulatory factor (IRF)‐binding ISRE element, maintains the maximum activity. Mutation of the GT‐Box and IRSE element dramatically reducesAPOL1 promoter activity. EMSA and chromatin immunoprecipitation assays reveal that the transcription factors Sp1, IRF1, and IRF2 could interact with their cognate binding sites on theAPOL1 promoter. Overexpression of Sp1, IRF1, and IRF2 increases promoter activity, leading to increasedAPOL1 mRNA and protein levels, while knockdown of Sp1, IRF1, and IRF2 has the opposite effects. These results demonstrate that theAPOL1 gene could be regulated by Sp1, IRF1, and IRF2 in hepatoma cells.
Source: FEBS Letters - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research