Effects of high relative humidity and dry purging on VOCs obtained during breath sampling on common sorbent tubes
Offline breath analysis by thermal desorption gas chromatography mass spectrometry (TD-GC-MS)
requires the use of sorbent traps to concentrate and store volatile compounds. The selection of
which sorbent to use and best practices for managing high relative humidity are important
considerations to allow for reproducible, untargeted, biomarker discovery in water saturated breath
samples. This work aims to assess three commonly used sorbent materials for their use in breath
volatile sampling and determine how the high relative humidity inherent in such samples effects the
capture of volatile compounds of interest. TenaxGR, TenaxTA/Carbograph1TD and TenaxTA/Carbograph5TD
tubes were selected as they are the most commonly used sorbents in the breath sampling literature.
The recovery of 29 compounds in a standard mix loaded using high humidity gas was tested for each
sorbent and compared to loading in dry gas. Water retention and dry purge rates were determined for
each sorbent for 500...
Source: Journal of Breath Research - Category: Respiratory Medicine Authors: Maxim Wilkinson, Iain R White, Royston Goodacre, Tamara Nijsen and Stephen J Fowler Source Type: research
More News: Respiratory Medicine