Generation and characterization of monoclonal antibodies specific for the Pseudorabies Virus nuclear egress complex.

Generation and characterization of monoclonal antibodies specific for the Pseudorabies Virus nuclear egress complex.
Virus Res. 2020 Jul 16;:198096
Authors: Hölper JE, Reiche S, Franzke K, Mettenleiter TC, Klupp BG
Abstract
During herpesvirus replication, newly synthesized nucleocapsids exit the nucleus by a vesicle-mediated transport, which requires the nuclear egress complex (NEC), composed of the conserved viral proteins designated as pUL31 and pUL34 in the alphaherpesviruses pseudorabies virus (PrV) and herpes simplex viruses. Oligomerization of the heterodimeric NEC at the inner nuclear membrane (INM) results in membrane bending and budding of virus particles into the perinuclear space. The INM-derived primary envelope then fuses with the outer nuclear membrane to release nucleocapsids into the cytoplasm. The two NEC components are necessary and sufficient for induction of vesicle budding and scission as shown after co-expression in eukaryotic cells or in synthetic membranes. However, where and when the NEC is formed, how membrane curvature is mediated and how it is regulated, remains unclear. While monospecific antisera raised against the different components of the PrV NEC aided in the characterization and intracellular localization of the individual proteins, no NEC specific tools have been described yet for any herpesvirus. To gain more insight into vesicle budding and scission, we aimed at generating NEC specific monoclona...

Source: Virus Research - July 15, 2020 Category: Virology Authors: Hölper JE, Reiche S, Franzke K, Mettenleiter TC, Klupp BG Tags: Virus Res Source Type: research