Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.

Effect of varied hair protein fractions on the gel properties of keratin/chitosan hydrogels for the use in tissue engineering.
Colloids Surf B Biointerfaces. 2020 Jul 13;195:111258
Authors: Lu TY, Huang WC, Chen Y, Baskaran N, Yu J, Wei Y
Abstract
Keratin/chitosan composite is a readily available source for a hybrid hydrogel in tissue engineering. While human hair keratins could provide biological functions, chitosan could further enhance the mechanical strength of the hybrid hydrogels. However, hair keratin is a group of natural proteins, and the uncontrolled hair protein contents in a hydrogel may lead to the batch-to-batch inconsistent gel properties. The purpose of this study was to investigate the role of hair protein composition, including the keratin-associated proteins (KAPs, 6-30 kDa) and keratin intermediate filaments (KIFs, 45-60 kDa) on gel characteristics of the keratin/chitosan hydrogel. The various compressive and tensile modulus of the gel was observed based on the selection of different protein fractions as the significant gel components. These results thus suggest a straightforward method of preparing hair keratin/chitosan hydrogel with much more controllable gel properties by merely modulating the KAPs/KIFs ratios in a gel.
PMID: 32683238 [PubMed - as supplied by publisher]

Source: Colloids and Surfaces - July 12, 2020 Category: Biotechnology Authors: Lu TY, Huang WC, Chen Y, Baskaran N, Yu J, Wei Y Tags: Colloids Surf B Biointerfaces Source Type: research