Analysis of RNA-DNA Triplex Structures In Vitro and In Vivo.
Analysis of RNA-DNA Triplex Structures In Vitro and In Vivo.
Methods Mol Biol. 2020;2161:229-246
Authors: Postepska-Igielska A, Blank-Giwojna A, Grummt I
Abstract
RNA can bind within the major groove of purine-rich DNA via Hoogsteen base pairing and form a triple helical RNA-DNA structure that anchors the RNA to specific DNA sequences, thereby targeting RNA-associated regulatory proteins to distinct genomic sites. Here we present methods to analyze the potential of a given RNA to form triplexes in vitro and to validate these structures in vivo.
PMID: 32681516 [PubMed - as supplied by publisher]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Postepska-Igielska A, Blank-Giwojna A, Grummt I Tags: Methods Mol Biol Source Type: research
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