Immunohistochemical Study of Tubulovillous Colon Adenoma in the Elderly

AbstractThe incidence of tubulovillous adenoma is higher than that of colorectal cancer. Most adenomas are likely prone to malignancy. It is often impossible to identify the adenomas that are prone to neoplasia. However, an increased adenoma size and severe dysplasia contribute to malignancy. The purpose of the study was to evaluate cell proliferation, apoptosis, and β-catenin in tubulovillous colon adenoma with varying degrees of dysplasia. Biopsy specimens of tubulovillous adenoma from 50 patients who underwent surgical removal were used. After resection, the adenomas were cut to the largest size so that the entire thickness oftunicae mucosae andmuscularis was included in the section. Markers of cell proliferation (Ki-67), apoptosis (P-53), and transcription factor ( β-catenin) were used for immunohistochemical reactions. In tubulovillous adenoma with low-grade dysplasia, there are low-level indicators of cell proliferation and apoptosis located mainly in the glandular basal segments. As the degree of dysplasia increases, cellular division in the glandular epit helium increases, and nuclear expression of β-catenin appears. A small number of cells in an apoptotic state were detected against the background of significant increase in cell proliferation. Thus, an increase in the Ki-67 and β-catenin indicators in tubulovillous adenoma with high-grade dysplasi a helps to limit the cellular differentiation and disrupts intercellular contacts.

Source: Advances in Gerontology - March 31, 2020 Category: Geriatrics Source Type: research