Identification and structural characterization of lipid A from Escherichia coli, Pseudomonas putida and Pseudomonas taiwanensis by liquid chromatography coupled to high resolution-tandem mass spectrometry.

CONCLUSIONS: The hyphenation of HPLC and MS/MS enabled the selective and sensitive identification and structural characterization of various lipid A species from different gram-negative bacteria. These species varied in their substituted side chains, speaking of fatty acids and phosphate groups. Characteristic product ions facilitated the assignment of side chains to distinct positions of the lipid A backbone. PMID: 32673427 [PubMed - as supplied by publisher]
Source: Rapid Communications in Mass Spectrometry : RCM - Category: Chemistry Authors: Tags: Rapid Commun Mass Spectrom Source Type: research