SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro.

SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro.
Mem Inst Oswaldo Cruz. 2020;115:e200200
Authors: Cipitelli MDC, Valentin E, da Cruz NVG, Nogueira TL, de Melo ECA, da Silva RA, Serra MM, Meriano AL, Colares AM, Dornelas-Ribeiro M, Santos CG
Abstract
Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.
PMID: 32667461 [PubMed - as supplied by publisher]

Source: Memorias do Instituto Oswaldo Cruz - July 16, 2020 Category: Infectious Diseases Authors: Cipitelli MDC, Valentin E, da Cruz NVG, Nogueira TL, de Melo ECA, da Silva RA, Serra MM, Meriano AL, Colares AM, Dornelas-Ribeiro M, Santos CG Tags: Mem Inst Oswaldo Cruz Source Type: research