The golgin family exhibits a propensity to form condensates in living cells

AbstractThe Golgi is surrounded by a ribosome ‐excluding matrix. Recently, we reported that the cis‐Golgi‐localized golgin GM130 can phase‐separate to form dynamic, liquid‐like condensatesin vitro andin vivo. Here, we show that overexpression of each of the remaining cis (golgin160, GMAP210) and trans (golgin97, golgin245, GCC88, GCC185) golgins results in novel protein condensates. Focused ion beam scanning electron microscopy (FIB ‐SEM) images of GM130 condensates reveal a complex internal organization with branching aqueous channels. Pairs of golgins overexpressed in the same cell form distinct juxtaposed condensates. These findings support the hypothesis that, in addition to their established roles as vesicle tethers, pha se separation may be a common feature of the golgin family that contributes to Golgi organization.

Source: FEBS Letters - July 14, 2020 Category: Biochemistry Authors: Pascal Ziltener, Aleksander A. Rebane, Morven Graham, Andreas M. Ernst, James E. Rothman Tags: RESEARCH LETTER Source Type: research