RECK and TIMP-2 mediate inhibition of MMP-2 and MMP-9 by Annona muricata.

RECK and TIMP-2 mediate inhibition of MMP-2 and MMP-9 by Annona muricata. J Biosci. 2020;45: Authors: Drishya G, Nambiar J, Shaji SK, Vanuopadath M, Achuthan A, Kumar A, Alias A, Sherif A, Joseph C, Divya P, Kumar DS, Bose C, Nair SV, Sudarslal S, Kumar GB, Lakshmi S, Nair BG Abstract Up-regulation of MMP-2 and MMP-9 plays a significant role in promoting cancer progression by degrading the components of the extracellular matrix, thereby enhancing the migration of tumor cells. Although the antiproliferative and apoptotic effect of Annona muricata is well established, its effect on MMP-2 and MMP-9, a major target in several types of cancers, has not been studied. Powdered samples of various parts of A. muricata like fruit, stem, seed, and twig extracted using aqueous methanol showed significant dose-dependent inhibition of MMP-2 and MMP-9 in a highly metastatic fibrosarcoma cell line, HT1080. Additionally, these extracts also up-regulated the expression of several endogenous inhibitors of MMP-2 and MMP-9 like REversion-inducing Cysteine-rich protein with Kazal motifs (RECK) and Tissue Inhibitor of Metalloproteinase- 2 (TIMP-2). Furthermore, primary cells developed from tumor tissues obtained from patients not exposed to chemotherapy, also exhibited similar results. Remarkably, the inhibition of MMP-2 and MMP-9 observed was tumor specific, with the A. muricata fruit extract showing only 2% inhibition in cells obtained from normal tissue...
Source: Journal of Biosciences - Category: Biomedical Science Authors: Tags: J Biosci Source Type: research