Long non-coding RNA AWPPH enhances malignant phenotypes in nasopharyngeal carcinoma via silencing PTEN through interacting with LSD1 and EZH2.

In this study, AWPPH expression was markedly upregulated in NPC cells. Besides, the loss- and gain-of-function assays indicated that AWPPH facilitated cell proliferation and migration while restrained cell apoptosis in NPC cells. Moreover, cytoplasmic AWPPH was predicted to share a common RNA-binding protein, IGF2BP1, with LSD1. And the interaction of IGF2BP1 with both AWPPH and LSD1 mRNA was certified in NPC cells, while AWPPH stabilized LSD1 mRNA to enhance the expression of LSD1 in NPC through such interaction. Furthermore, nuclear AWPPH repressed PTEN expression through recruiting EZH2 and LSD1 to PTEN promoter in NPC cells. Final rescue assays demonstrated that silenced PTEN could reverse the suppressive influence of AWPPH depletion on NPC progression. Collectively, our study manifested that AWPPH inhibited PTEN expression to drive NPC progression through interacting with LSD1 and EZH2, providing potential biomarkers for NPC treatment.
PMID: 32663416 [PubMed - as supplied by publisher]

Source: Biochemistry and Cell Biology - July 13, 2020 Category: Biochemistry Authors: Guo D, Liu F, Zhang L, Bian N, Liu L, Kong L, Hu Q, Wang Z Tags: Biochem Cell Biol Source Type: research