Lysosome Associated Protein Transmembrane 4B (LAPTM4B)-24 is the predominant protein isoform in human tissues and undergoes rapid, nutrient-regulated turnover

Studies of Lysosome Associated Protein Transmembrane 4B (LAPTM4B) have mainly focused on the 35 kDa isoform and its association with poor prognosis in cancers. Here, by employing a novel monoclonal antibody we found that the 24 kDa LAPTM4B isoform predominated in most, both healthy and malignant, human cells and tissues studied. LAPTM4B-24 lacks the extreme N-terminus and contrary to LAPTM4B-35, failed to promote cell migration. The endogenous LAPTM4B-24 protein was subject to rapid turnover with a t1/2 ∼1 h.
Source: American Journal of Pathology - Category: Pathology Authors: Tags: Regular Article Source Type: research