In silico analysis of a chimeric fusion protein as a new vaccine candidate against Clostridium perfringens type A and Clostridium septicum alpha toxins

AbstractIn silico analysis is the most important approach to understand protein structure and functions, and the most important problem for designing and producing a fusion construct is producing large amounts of functional protein.Clostridium perfringens type A andClostridium septicum produce alpha (plc) and alpha toxins respectively.C. perfringens can cause gas gangrene and gastrointestinal diseases.C. septicum can cause traumatic and non-traumatic gas gangrene. The aim of current research was in silico analysis of a chimeric fusion protein againstC. perfringens type A andC. septicum alpha toxins. Firstly, the chimeric fusion gene was designed according to nucleotide sequences ofC. perfringens type A alpha (KY584046.1) andC. septicum alpha (JN793989.2) toxin genes and then its fusion protein is constructed by amino acid sequences ofC. perfringens type A andC. septicum alpha toxins. Secondly, online software was used to determine prediction of secondary and tertiary structures and physicochemical characteristics of the fusion protein. Finally, the validation of the fusion protein was confirmed by Rampage and proSA program. The designed fusion protein has 777 amino acids in length. TASSER server and physicochemical parameters are showed: C-score  = − 2.68 and molecular weight = 87.9 KD respectively. Rampage and proSA software revealed the fusion protein is valid. Deposited accession number for the sequence of the fusion gene in the GenBank is MK908396. The design...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research