VcMYB4a, an R2R3-MYB transcription factor from Vaccinium corymbosum, negatively regulates salt, drought, and temperature stress.

In this study, we cloned a novel MYB TF gene from Vaccinium corymbosum (blueberry) using rapid amplification of cDNA ends (RACE). The cDNA contained a 798-bp open reading frame that encodes a 265-amino acid protein. VcMYB4a possessed a C2/EAR-repressor motif domain and phylogenetic analysis showed that it clustered into a subgroup 4 with six Arabidopsis thaliana MYBs. Quantitative RT-PCR analysis demonstrated that VcMYB4a expression was downregulated by salt, drought, and cold treatment, but was induced by freezing and heat. Overexpression of VcMYB4a in blueberry callus enhanced sensitivity to salt, drought, cold, freezing, and heat stress. These results indicate that VcMYB4a may be an important repressor of abiotic stress in blueberry. PMID: 32653482 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/32653482?dopt=Abstract

Source: Gene - July 8, 2020 Category: Genetics & Stem Cells Authors: Zhang CY, Liu HC, Zhang XS, Guo QX, Bian SM, Wang JY, Zhai LL Tags: Gene Source Type: research