In vivo [U-13C]-glucose labeling to assess heart metabolism in murine models of pressure and volume overload.
In vivo [U-13C]-glucose labeling to assess heart metabolism in murine models of pressure and volume overload.
Am J Physiol Heart Circ Physiol. 2020 Jul 10;:
Authors: Schnelle M, Chong M, Zoccarato A, Elkenani M, Sawyer GJ, Hasenfuss G, Ludwig C, Shah AM
Abstract
Alterations in the metabolism of substrates such as glucose are integrally linked to the structural and functional changes that occur in the remodeling heart. Assessment of such metabolic changes under in vivo conditions would provide important insights into this inter-relationship. We aimed to investigate glucose carbon metabolism in pressure-overload and volume-overload cardiac hypertrophy by using an in vivo [U-13C]-glucose labeling strategy to enable analyses of the metabolic fates of glucose carbons in the mouse heart. Therefore, [U-13C]-glucose was administered in anesthetized mice by tail vein infusion and the optimal duration of infusion established. Hearts were then excised for 13C metabolite isotopomer analysis by NMR spectroscopy. [U-13C]-glucose infusions were performed in mice 2 weeks following transverse aortic constriction (TAC) or aorto-caval fistula (Shunt) surgery. At this time point, there were similar increases in left ventricular (LV) mass in both groups but TAC resulted in concentric hypertrophy with impaired LV function whereas Shunt caused eccentric hypertrophy with preserved LV function. TAC was accompanied by significant changes in glycolysis, mitoch...
Source: American Journal of Physiology. Heart and Circulatory Physiology - Category: Physiology Authors: Schnelle M, Chong M, Zoccarato A, Elkenani M, Sawyer GJ, Hasenfuss G, Ludwig C, Shah AM Tags: Am J Physiol Heart Circ Physiol Source Type: research