An off-on electrochemiluminescence detection for microRNAs based on TiO2 nanotubes sensitized with gold nanoparticles as enhanced emitters.

An off-on electrochemiluminescence detection for microRNAs based on TiO2 nanotubes sensitized with gold nanoparticles as enhanced emitters. Anal Bioanal Chem. 2020 Jul 09;: Authors: Dai P, Ke J, Xie C, Wei L, Zhang Y, He Y, Chen L, Jin J Abstract A sensitive electrochemiluminescence (ECL) assay for microRNAs (miRNAs) based on a semiconductor nanomaterial sensitized with noble-metal Au nanoparticles (NPs) is successfully developed. TiO2 nanotubes (NTs) were equipped with Au NPs to obtain an enhanced ECL emitter. Then, an ECL assay for miRNA-21 was fabricated, which was based on the use of probe 2 DNA-functionalized Pt/PAMAM nanocomposites (NCs) assembled on the surface of Au/TiO2 NT conjugate via DNA hybridization between probe 1 DNA and capture DNA. The Pt/PAMAM NCs act as an ECL quencher of Au/TiO2 NTs via resonance energy transfer. After the binding of target miRNA-21 and the capture DNA, the Pt/PAMAM NCs were released and the ECL signal was recovered. An "off-on" ECL assay was achieved with a linear response from 0.01 to 10,000 pM. Finally, this method has been validated to be sensitive and specific for miRNAs in human serum samples. The ECL enhancement strategy opens a new way for fabricating various sensitive biosensors. Graphical abstract A sensitive "off-on" electrochemiluminescence analysis method was developed, which combined Au NP-enhanced ECL emission of TiO2 nanotubes and an efficient energy-transfer system between Au/Ti...
Source: Analytical and Bioanalytical Chemistry - Category: Chemistry Authors: Tags: Anal Bioanal Chem Source Type: research