Resveratrol and celastrol loaded Collagen dental implants regulate periodontal ligament fibroblast growth and osteoclastogenesis of bone marrow macrophages.

Resveratrol and celastrol loaded Collagen dental implants regulate periodontal ligament fibroblast growth and osteoclastogenesis of bone marrow macrophages. Chem Biodivers. 2020 Jul 10;: Authors: Wu W, Wang R, Bao B, Bao C, Wang S, Rahman SU, Hou C, Elango J Abstract Collagen is widely used for dental therapy in several ways such as films, 3D matrix, and composites, besides traditional Chinese medicine (TCM) has been used in tissue regeneration and wound healing application for centuries. Hence, the present study was targeted for the first time to fabricate collagen film with TCM such as resveratrol and celastrol in order to investigate the human periodontal ligament fibroblasts (HPLF) growth and bone marrow macrophages (BMM) derived osteoclastogenesis. Further, the physicochemical, mechanical and biological activities of collagen-TCMfilms crosslinked by glycerol and EDC-NHS (1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide-N-hydroxysulfosuccinimide) were investigated. Collagen film characterization was significantly regulated by the nature of plasticizers like hydrophobic and degree of polarity. Interestingly, the collagen film'sdenaturation temperature was increased by EDC-NHS than glycerol. FTIR data confirmed the functional group changes due to chemical interaction of collagen with TCM. Morphological changes of HPLF cells cultured in control and collagen films were observed by SEM. Importantly, the addition of resveratrol upregulat...
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Tags: Chem Biodivers Source Type: research