MARCKS-Like Protein-1(MLP-1) regulates ENaC Activity in Renal Distal Convoluted Tubule Cells.

We examined MLP-1 regulation of ENaC in DCT-15 cells. MLP-1 consists of a positively charged effector domain that sequesters PIP2 and contains serines that are a PKC targets and controls MLP-1 membrane association; a myristoylation domain promoting membrane association, and a multiple homology 2 domain of unknown function. We constructed several MLP-1 mutants: WT: a full length wild-type protein; S3A: 3 substitutions in the effector domain to prevent phosphorylation; S3D: replaced three serines with aspartates to mimic constitutive phosphorylation; GA: replaced the myristoylation site glycine with alanine so GA could not be myristoylated. Mutants were tagged with N-terminal 3XFLAG or C-terminal m-Cherry or V5. Transfection with MLP mutants modified ENaC activity: S3A activity was highest and S3D lowest; the activity of both was significantly different from WT. In Western blots, when transfected with 3XFLAG tagged MLP-1 mutants, expression of full length MLP-1 at 52 KDa increased in S3A-MLP-1 transfected cells and decreased in S3D-MLP-1 transfected cells. Lower molecular weight bands were detected that correspond to potential protease cleavage products. Confocal imaging shows that mutants localize in different sub-cellular compartments consistent with their preferred location in the membrane or cytosol. Protein kinase C increases phosphorylation of endogenous MLP-1 and reduces ENaC activity. Our results suggest a complicated role for proteolytic processing in MLP-1 regulation ...

Source: Am J Physiol Cell Ph... - July 7, 2020 Category: Cytology Authors: Song C, Yue Q, Moseley A, Al-Khalili O, Wynne BM, Ma H, Wang L, Eaton DC Tags: Am J Physiol Cell Physiol Source Type: research