How to rapidly and sensitively detect for Escherichia coli O157:H7 and Salmonella Typhimurium in cabbage using filtration, DNA concentration, and real-time PCR after short-term enrichment

Publication date: Available online 9 July 2020Source: LWTAuthor(s): Seung-Hae Gwak, Jin-Hee Kim, Se-Wook Oh
Source: LWT Food Science and Technology - Category: Food Science Source Type: research

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dra Combrinck Terminalia sericea Burch. ex. DC. (Combretaceae) is a popular remedy for the treatment of infectious diseases. It is widely prescribed by traditional healers and sold at informal markets and may be a good candidate for commercialisation. For this to be realised, a thorough phytochemical and bioactivity profile is required to identify constituents that may be associated with the antibacterial activity and hence the quality of raw materials and consumer products. The aim of this study was to explore the phytochemistry and identify the antibacterial constituents of T. sericea root bark, using a metabolomic a...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Publication date: October 2020Source: LWT, Volume 132Author(s): Tofa Begum, Peter A. Follett, Farah Hossain, Larry Christopher, Stephane Salmieri, Monique Lacroix
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
The SlyA transcriptional regulator controls the expression of genes involved in virulence and production of surface components in S. Typhimurium and E. coli. Its mode of action is mainly explained by its antagonism with the H-NS repressor for the same DNA binding regions. Interestingly, it has been reported that the alarmone ppGpp promotes SlyA dimerization and DNA binding at the promoter of pagC, enhancing the expression of this gene in Salmonella. A recurring problem in the field of stringent response has been to find a way of following ppGpp levels in vivo in real time. We thought that SlyA, as a ppGpp responsive ligand...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract One promising strategy to combat antibiotic-resistant bacteria is to develop compounds that block bacterial defenses against antibacterial conditions produced by the innate immune system. Salmonella enterica , which causes food-borne gastroenteritis and typhoid fever, requires histidine kinases to resist innate immune defenses such as cationic antimicrobial peptides (CAMPs). Here, we report that 2-aminobenzothiazoles block histidine kinase-dependent phenotypes in Salmonella enterica serotype Typhimurium. We found that 2-aminobenzothiazoles inhibited growth in low Mg 2+ , a stressful condition that require...
Source: Chembiochem - Category: Biochemistry Authors: Tags: Chembiochem Source Type: research
Publication date: November 2020Source: LWT, Volume 133Author(s): Cuauhtemoc Marin, Diana Ibañez, Gabriela Rios-Corripio, Jose Angel Guerrero, Dolores Rodrigo, Antonio Martinez
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Abstract Three new neoflavonoids, named (1S,8R,9S)-1,5-dihydroxy-4,12-dimethoxy-8-vinyl-tricyclo[,7]trideca-2,4,6,11-tetraen-10-one (1), 2,5,2',5'-tetrahydroxy-4-methoxybenzophenone (2) and 2,5,3'-trihydroxy-4-methoxybenzophenone (3), were isolated from the heartwood of Dalbergia melanoxylon. Their structures were established by spectroscopic methods, including UV, IR, HRMS, 1 D and 2 D-NMR. Compounds 1-3 were evaluated for inhibitory activity against three fungal strains Candida albicans, Mucor ramosissimus, Saccharomycopsis fermentans and seven bacterial strains Shigella dysenteriae, Salmon...
Source: Natural Product Research - Category: Biochemistry Authors: Tags: Nat Prod Res Source Type: research
Abstract Cell blocking (CB) technique has been widely applied in many studies since the last century. In our research, this technique was mostly used to study the enhancement of the vacuolar response-based system that could detect Shigella sp. and Salmonella sp. investigated in previous studies. The recombinant yeast cells were blocked by mixing with agarose gel on a 96-wells plate, then storing this plate in -80 °C before using. The optimal conditions for the new system, such as agarose concentration, maximum storage time, were also established. Finally, the efficiency of the vacuolar response-based syst...
Source: Analytical Biochemistry - Category: Biochemistry Authors: Tags: Anal Biochem Source Type: research
Abstract Salmonella infection predominantly causes four clinical syndromes: enteric fever, gastroenteritis, bacteremia, and asymptomatic carrier state. Salmonella osteomyelitis is an extremely rare manifestation of salmonella infection except in children with hemoglobinopathies. Salmonella osteomyelitis has been reported to mostly affect the diaphysis of long bones and lumbar spine. Here, we describe a case of salmonella osteomyelitis of the right 6th rib in a 74-year-old woman who presented with breast pain, swelling, high fever and local heat. Her medical history showed myocardial infarction; namely, at the age ...
Source: The Tohoku Journal of Experimental Medicine - Category: Research Authors: Tags: Tohoku J Exp Med Source Type: research
We describe the experimental procedure and precautions for the production of this molybdo-enzyme. StMsrP was also demonstrated to reduce free Met-O and to catalyse the complete repair of an oxidized protein. More importantly, this study provides for the first time, access to the exhaustive list of the Msr systems of a pathogen, including four cytoplasmic enzymes (MsrA, MsrB, MsrC, BisC) and one periplasmic enzyme(MsrP). PMID: 32750406 [PubMed - as supplied by publisher]
Source: Free Radical Biology and Medicine - Category: Biology Authors: Tags: Free Radic Biol Med Source Type: research
Salmonellosis is an important food borne disease of public health significance. Global estimates of the disease burden shows more than 20 million cases and 0.15 million deaths annually. The disease caused by a variety ofSalmonella organisms worldwide.Salmonella pathogens are belonging to family Enterobacteriaceae that are known to infect many hosts inducing variable clinical diseases pictures. Typhoidal and non-typhoidalSalmonellae are common diseases among Egyptians with severe socioeconomic losses. Different species of animals and poultry as well as their products are the main sources and reservoirs for zoonotic human il...
Source: The Journal of Infection in Developing Countries - Category: Infectious Diseases Source Type: research
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